منابع مشابه
Second-harmonic imaging microscopy of living cells.
Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce three-dimensional...
متن کاملSecond-harmonic imaging microscopy of normal human and keratoconus cornea.
PURPOSE The purpose of this study was to evaluate the ability of second-harmonic imaging to identify differences in corneal stromal collagen organization between normal human and keratoconus corneas. METHODS Six normal corneas from eye bank donors and 13 corneas of patients with keratoconus, obtained after penetrating keratoplasty were examined. A femtosecond titanium-sapphire laser with 800-...
متن کاملInterferometric second harmonic generation microscopy.
We describe a novel second harmonic generation (SHG) microscope that employs heterodyne detection by interfering the epi directed SHG from a sample with SHG from a reference crystal. In addition, the microscope provides complementary reflectance information based on optical coherence microscopy (OCM). The instrument features dual balanced detection to minimize the effect of source fluctuations,...
متن کاملTwo Photon Microscopy and Second Harmonic Generation
Nonlinear microscopy has become an important tool for imaging biological samples such as neurons. In two photon fluorescence, a fluorophore absorbs two photons at a low energy state, one of its electrons briefly converts to a higher energy state, and it emits a single excited photon at an energy level higher than that of the absorbed photons. In second harmonic generation (SHG), laser light is ...
متن کاملSecond-harmonic imaging of collagen.
Molecules that have no center of symmetry are able to convert light to its second harmonic, at twice the frequency and half the wavelength. This only happens with any efficiency at very high light intensities such as are given by a pulsed laser, and because the efficiency of the process depends on the square of the intensity, it will be focal plane selective in exactly the same way as two-photo...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2003
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s143192760344066x